Triazole Fungicides Inhibit Zebrafish Hatching by Blocking the Secretory Function of Hatching Gland Cells. (Miguel L. Allende et al, 2017).
Locomotor Activity Quantification in zebrafish larvae exposed to the agrochemical “triadimefon” (FON).
In this bioassay a triazole family fungicide is evaluated in order to establish the effects of the compound on the behaviour of zebrafish larvae 3-dpf.
To assay locomotor activity, 3 dpf larvae were incubated in a 96-well multiwell plate with 250 _L
of FON 16 mg/L at 28 _C during 8 of light.
Triadimefon increases locomotor activity (LMA) in 3 dpf larvae. Larvae incubated with the
medium FON concentration show a significant increase in LMA when they are compared with
non-treated animals. The hyperactivity appears after 3 h of incubation and persists until at least
Int J Mol Sci. 2017 Apr 4;18(4). pii: E710. doi: 10.3390/ijms18040710.
Data are presented as average ± SEM from 32 to 40 larvae per condition from four
independent experiments. Comparisons were performed using two-way ANOVA, with Sidak’s
post-test (statistical significance is compared with controls pared with controls * p < 0.05, ** p <