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Locomotor Activity Quantification in zebrafish larvae exposed to the agrochemical “triadimefon” (FON).

In this bioassay a triazole family fungicide is evaluated in order to establish the effects of the compound on the behaviour of zebrafish larvae 3-dpf.

To assay locomotor activity, 3 dpf larvae were incubated in a 96-well multiwell plate with 250 _L

of FON 16 mg/L at 28 _C during 8 of light.

Triadimefon increases locomotor activity (LMA) in 3 dpf larvae. Larvae incubated with the

medium FON concentration show a significant increase in LMA when they are compared with

non-treated animals. The hyperactivity appears after 3 h of incubation and persists until at least

seven hours.

Int J Mol Sci. 2017 Apr 4;18(4). pii: E710. doi: 10.3390/ijms18040710.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412296/

Triazole Fungicides

Data are presented as average ± SEM from 32 to 40 larvae per condition from four

independent experiments. Comparisons were performed using two-way ANOVA, with Sidak’s

post-test (statistical significance is compared with controls pared with controls * p < 0.05, ** p <

More: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412296/

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